abstract：:The advent of quantitative PCR has improved the detection of human viral pathogens in the environment. However, a serious limitation of this method may arise from the inability to discriminate between viruses that are infectious and viruses that have been inactivated and do not represent a human health hazard. To asse...

journal_title：Journal of virological methods

authors： Fittipaldi M,Rodriguez NJ,Codony F,Adrados B,Peñuela GA,Morató J

更新日期：2010-09-01 00:00:00

abstract：:A new system for inoculation of plants with begomoviral DNA without cloning or the use insect vectors is described. Total DNA extracted from begomovirus-infected plants was amplified by rolling circle amplification (RCA) using the bacteriophage phi29 DNA polymerase, and inoculated to plants by particle bombardment. In...

journal_title：Journal of virological methods

authors： Guenoune-Gelbart D,Sufrin-Ringwald T,Capobianco H,Gaba V,Polston JE,Lapidot M

更新日期：2010-09-01 00:00:00

abstract：:Endothelial cell tropism is an inherent property of isolates of human cytomegalovirus (HCMV), and has been proposed as a pathogenicity factor of HCMV. Mutational approaches can be applied for analysis of the molecular determinants of endothelial cell tropism, but the evaluation of mutants is limited by the low reliabi...

journal_title：Journal of virological methods

authors： Schuessler A,Sampaio KL,Sinzger C

更新日期：2010-08-01 00:00:00

abstract：:Reverse genetics system is a powerful tool to study the function of a particular gene. The currently available reverse genetics system for Novirhabdovirus is based on vaccinia-driven T7 RNA polymerase expression. An improved system for the recovery of infectious hematopoietic necrosis virus (IHNV) was developed which ...

journal_title：Journal of virological methods

authors： Ammayappan A,Lapatra SE,Vakharia VN

更新日期：2010-08-01 00:00:00

abstract：:Loop-mediated isothermal amplification (LAMP) is a nucleic acid amplification method applied and adapted to the detection of a number of pathogens. A LAMP assay was developed for the specific detection of wild-type classical swine fever virus (CSFV). Based on an alignment of genomic sequences of pestiviruses available...

journal_title：Journal of virological methods

authors： Zhang XJ,Sun Y,Liu L,Belák S,Qiu HJ

更新日期：2010-07-01 00:00:00

abstract：:Wild birds have been implicated in the spread of highly pathogenic avian influenza (HPAIV) of the H5N1 subtype, prompting surveillance along migratory flyways. Sampling of wild birds is often conducted in remote regions, but results are often delayed because of limited local analytical capabilities, difficulties with ...

journal_title：Journal of virological methods

authors： Takekawa JY,Iverson SA,Schultz AK,Hill NJ,Cardona CJ,Boyce WM,Dudley JP

更新日期：2010-06-01 00:00:00

abstract：:In ovo vaccination remains an attractive option for a cost effective, uniform and mass application of vaccines for commercial poultry. However, the vaccines which can be delivered safely by this method are limited and there is no currently licensed embryo-safe vaccine against infectious bronchitis virus (IBV). In this...

journal_title：Journal of virological methods

authors： Zeshan B,Zhang L,Bai J,Wang X,Xu J,Jiang P

更新日期：2010-06-01 00:00:00

abstract：:Newcastle disease virus (NDV), also designated avian paramyxovirus type 1 (APMV-1), is a serious pathogen of poultry, causing highly contagious Newcastle disease (ND), with high morbidity or mortality, depending on the strain. Accordingly, rapid and reliable detection of APMV-1 and differentiation between vaccine and ...

journal_title：Journal of virological methods

authors： Fratnik Steyer A,Rojs OZ,Krapez U,Slavec B,Barlic-Maganja D

更新日期：2010-06-01 00:00:00

abstract：:Hepatitis A virus (HAV) infection is the leading worldwide cause of acute viral hepatitis. An important aspect of viral control is rapid diagnosis. Epidemiological studies have linked hepatitis A outbreaks to the consumption of drinking water or soft fruits exposed to faecal contamination. Real-time reverse transcript...

journal_title：Journal of virological methods

authors： Blaise-Boisseau S,Hennechart-Collette C,Guillier L,Perelle S

更新日期：2010-06-01 00:00:00

abstract：:Vaccine manufacturing requires constant analytical monitoring to ensure reliable quality and a consistent safety profile of the final product. Concentration and bioactivity of active components of the vaccine are key attributes routinely evaluated throughout the manufacturing cycle and for product release and dosage. ...

journal_title：Journal of virological methods

authors： Joelsson D,Gates IV,Pacchione D,Wang CJ,Bennett PS,Zhang Y,McMackin J,Frey T,Brodbeck KC,Baxter H,Barmat SL,Benetti L,Bodmer JL

更新日期：2010-06-01 00:00:00

abstract：:The first case of pandemic influenza A/H1N1/2009 infection was reported in Mexico in mid-April, 2009, and to date the new H1N1 virus has spread to over 160 countries. Therefore, it is important to obtain reliable epidemiological data on the spread of this virus by novel molecular methods can be developed in detection ...

journal_title：Journal of virological methods

authors： Wu W,Kang X,Bai Z,Liu L,Li J,Wu X,Sun H,Hu T,Yang M,Wang P,Liu L,Yang Y,Di B,Chen W

更新日期：2010-05-01 00:00:00

abstract：:The Cucumber mosaic virus (CMV) is an isodiametric plant virus with an extremely wide host range, present worldwide. CMV chimeric particles (R9-CMV), engineered to express a 27-aa synthetic peptide derived from Hepatitis C virus (HCV), were demonstrated to be stable under simulated gastric and intestinal conditions. T...

journal_title：Journal of virological methods

authors： Nuzzaci M,Vitti A,Condelli V,Lanorte MT,Tortorella C,Boscia D,Piazzolla P,Piazzolla G

更新日期：2010-05-01 00:00:00

abstract：:The aim of this study was the development of gag and pol dual labelled probe real-time PCR and RT PCR assays to quantify the proviral load and the transcripts of the British Visna/maedi virus EV1 strain. Primers and probes were chosen based on the consensus sequences of gag and pol clones representative of EV1 genetic...

journal_title：Journal of virological methods

authors： Carrozza ML,Mazzei M,Bandecchi P,Fraisier C,Pérez M,Suzan-Monti M,de Andrés D,Amorena B,Rosati S,Andrésdottir V,Lujan L,Pepin M,Blacklaws B,Tolari F,Harkiss GD

更新日期：2010-05-01 00:00:00

abstract：:A real-time PCR assay, which enables simultaneous detection and differentiation of all three serotypes of Marek's disease virus, without the need for post-PCR sequencing, has been developed. The assay is based on the primer-probe energy transfer real-time PCR, which has a relatively high tolerance towards point mutati...

journal_title：Journal of virological methods

authors： Barfoed AM,Østergaard E,Frandsen PL,Nielsen EB,Sandberg E,Rasmussen TB

更新日期：2010-04-01 00:00:00

abstract：:The diagnosis of acute hepatitis E infection is based on the detection of HEV RNA or specific IgM in immunocompetent patients. Viraemia and excretion of HEV RNA in faeces are not observed in all patients and commercial kits vary in their performance for anti-HEV IgM detection. Additional diagnostic tests must therefor...

journal_title：Journal of virological methods

authors： Bigaillon C,Tessé S,Lagathu G,Nicand E

更新日期：2010-03-01 00:00:00

abstract：:A multiplex real-time PCR assay was developed to detect and quantify the Citrus tristeza virus (CTV) genotypic mixture present in infected plants. CTV isolate FS627, a complex Florida isolate containing T36, T30 and VT genotypes and its aphid transmitted subisolates was used. The relative quantitative assay was carrie...

journal_title：Journal of virological methods

authors： Ananthakrishnan G,Venkataprasanna T,Roy A,Brlansky RH

更新日期：2010-03-01 00:00:00

abstract：:Allele-specific PCR based on subtype consensus sequences is a powerful technique for detecting low frequency drug resistant mutants in HIV-1 infected patients. However, this approach can be limited by genetic variation in the region complementary to the primers, leading to variability in allele detection. The goals of...

journal_title：Journal of virological methods

authors： Boltz VF,Maldarelli F,Martinson N,Morris L,McIntyre JA,Gray G,Hopley MJ,Kimura T,Mayers DL,Robinson P,Mellors JW,Coffin JM,Palmer SE

更新日期：2010-03-01 00:00:00

abstract：:Rapid detection of novel swine origin influenza A virus (S-OIV) (H1N1) is crucial for timely implementation of infection control measures. In this study, a haemagglutinin (HA) gene-based real-time nucleic acid sequence-based amplification (NASBA) assay was developed for the specific detection of S-OIV (H1N1). The assa...

journal_title：Journal of virological methods

authors： Ge Y,Cui L,Qi X,Shan J,Shan Y,Qi Y,Wu B,Wang H,Shi Z

更新日期：2010-02-01 00:00:00

abstract：:A lateral flow device (LFD) for the detection of swine vesicular disease (SVD) virus (SVDV) and differential diagnosis from foot-and-mouth disease (FMD) was developed using a monoclonal antibody (Mab C70). The performance of the LFD was evaluated in the laboratory on suspensions of vesicular epithelia and cell culture...

journal_title：Journal of virological methods

authors： Ferris NP,Nordengrahn A,Hutchings GH,Paton DJ,Kristersson T,Merza M

更新日期：2010-02-01 00:00:00

abstract：:Porcine reproductive and respiratory syndrome virus (PRRSV) has a marked tropism for cells of the monocyte-macrophage lineage and accordingly, replicates in fully differentiated alveolar macrophages in the natural host. Despite the identification of several putative receptors for PRRSV on porcine alveolar macrophages ...

journal_title：Journal of virological methods

authors： Lee YJ,Park CK,Nam E,Kim SH,Lee OS,Lee du S,Lee C

更新日期：2010-02-01 00:00:00

abstract：:A viral entry assay where a beta-lactamase reporter protein fused to the influenza matrix protein-1 (BlaM1) is packaged as a structural component into influenza virus-like particles (VLPs) is described. The Bla reporter is released upon fusion with target cells and can be detected in live cells by flow cytometry, micr...

journal_title：Journal of virological methods

authors： Tscherne DM,Manicassamy B,García-Sastre A

更新日期：2010-02-01 00:00:00

abstract：:On June 11, 2009, the World Health Organization declared that the influenza A/H1N1/2009 virus had become the first influenza pandemic of the 21st century. Rapid detection and differentiation from seasonal and avian influenza would be beneficial for patient management and infection control. It was the aim of this study...

journal_title：Journal of virological methods

authors： Gunson R,Maclean A,Davies E,Bennett S,Miller R,Carman WF

更新日期：2010-02-01 00:00:00

abstract：:In March/April 2009, Mexico experienced an outbreak of respiratory illness, due to a new influenza of swine origin virus, which spread rapidly via human-to-human transmission, and became pandemic (A/H1N1pdm). Because of its unique genome composition, which includes gene segments of swine, avian and human origin, and t...

journal_title：Journal of virological methods

authors： Lalle E,Bordi L,Castilletti C,Meschi S,Selleri M,Carletti F,Lapa D,Travaglini D,Ippolito G,Capobianchi MR,Di Caro A

更新日期：2010-02-01 00:00:00

abstract：:A pandemic caused by a novel influenza A virus (H1N1) poses a serious public health threat. In this study, a real-time reverse transcriptase PCR (RT-PCR) assay based on the hemagglutinin gene was developed that discriminates the novel H1N1 from swine influenza virus, seasonal H1N1/H3N2 virus and the highly pathogenic ...

journal_title：Journal of virological methods

authors： Jiang T,Kang X,Deng Y,Zhao H,Li X,Yu X,Yu M,Qin E,Zhu Q,Yang Y,Qin C

更新日期：2010-02-01 00:00:00

abstract：:Indicator cell lines are useful biological tools for monitoring virus infection. In order to monitor infection with bovine immunodeficiency virus (BIV) in vitro, an indicator cell line derived from baby hamster kidney cells which contains integrated copies of an enhanced green fluorescent protein gene driven by the BI...

journal_title：Journal of virological methods

authors： Yao X,Su Y,Liu C,Tan J,Liu L,Geng YQ,Qiao WT

更新日期：2010-01-01 00:00:00

abstract：:The consumption of bivalve shellfish is a common cause of foodborne outbreaks of viral origin. The evaluation of the sanitary quality of these products, however, is still based on bacterial indicators of fecal contamination (Reg. (EC) No. 2073/2005 and No.1441/2007) even if it is known that they are not reliable indic...

journal_title：Journal of virological methods

authors： Di Pasquale S,Paniconi M,De Medici D,Suffredini E,Croci L

更新日期：2010-01-01 00:00:00

abstract：:Large-scale production of long dsRNA is needed if antiviral applications of RNAi are to succeed in shrimp farm operations. A novel hairpin-RNA expression vector was developed based on the RNA-dependent RNA polymerase (RdRp) gene of yellow head virus (YHV), the cause of a lethal shrimp disease. Using transformed RNase-...

journal_title：Journal of virological methods

authors： Saksmerprome V,Charoonnart P,Gangnonngiw W,Withyachumnarnkul B

更新日期：2009-12-01 00:00:00

abstract：:Recently, polyomaviruses KI and WU were identified in the airways of patients with acute respiratory symptoms. The epidemiology and pathogenesis of these two viruses are not fully understood, and the development of molecular assays, such as Real Time PCR, was useful for examining their biology and role in different cl...

journal_title：Journal of virological methods

authors： Bergallo M,Terlizzi ME,Astegiano S,Ciotti M,Babakir-Mina M,Perno CF,Cavallo R,Costa C

更新日期：2009-12-01 00:00:00

abstract：:Simian betaretroviruses (formerly Type D retroviruses; SRV) are a group of closely related retroviruses for which the natural host species are Asian monkeys of the genus Macaca. Five serotypes have been identified by classical neutralization assays and three additional untyped variants have been reported (SRV(Tsukuba)...

journal_title：Journal of virological methods

authors： White JA,Todd PA,Rosenthal AN,Yee JL,Grant R,Lerche NW

更新日期：2009-12-01 00:00:00

abstract：:Loop mediated isothermal amplification (LAMP) assay is used for rapid diagnosis of Cyprinid herpesvirus-3, formerly designated koi herpesvirus (KHV), with comparable sensitivity to PCR. To reduce the time required for the LAMP assay, an immunocapture (IC) and direct binding (DB) techniques were developed to exclude th...

journal_title：Journal of virological methods

authors： Soliman H,El-Matbouli M

更新日期：2009-12-01 00:00:00

abstract：:With the approval of the first HIV-1 integrase inhibitor raltegravir and a second one in phase III clinical development (elvitegravir), genotypic and phenotypic resistance assays are required to guide antiretroviral therapy and to investigate treatment failure. In this study, a genotypic and phenotypic recombinant vir...

journal_title：Journal of virological methods

authors： Van Baelen K,Rondelez E,Van Eygen V,Ariën K,Clynhens M,Van den Zegel P,Winters B,Stuyver LJ

更新日期：2009-11-01 00:00:00

abstract：:Henipaviruses were first discovered in the 1990s, and their potential threat to public health is of increasing concern with increasing knowledge. Old-world fruit bats are the reservoir hosts for these viruses, and spill-over events cause lethal infections in a wide range of mammalian species, including humans. In anti...

journal_title：Journal of virological methods

authors： Feldman KS,Foord A,Heine HG,Smith IL,Boyd V,Marsh GA,Wood JL,Cunningham AA,Wang LF

更新日期：2009-10-01 00:00:00

abstract：:The feasibility of detecting somatic coliphages by phage infection of Escherichia coli WG5 and measurement of phage propagation by the lysis mediated release of the bacterial host adenylate kinase (AK) and adenosine 5'-triphosphate (ATP) detected by a bioluminescent signal was evaluated. After 2h of incubation, all cu...

journal_title：Journal of virological methods

authors： Guzmán Luna C,Costán-Longares A,Lucena F,Jofre J

更新日期：2009-10-01 00:00:00

abstract：:Human immunodeficiency virus type-1 (HIV-1) RNA viral load is an important biomarker to evaluate the therapeutic efficacy of antiretroviral drugs and to monitor disease progression in HIV-infected individuals. We compared HIV-1 RNA quantitation between two different kits, the NucliSens EasyQ HIV-1 v1.1 (EasyQ, bioMéri...

journal_title：Journal of virological methods

authors： Choi JY,Kim EJ,Rho HJ,Kim JY,Kwon OK,Lee JH,Koo MJ,Kim SS

更新日期：2009-10-01 00:00:00

abstract：:The aim of this study was to evaluate the applicability of virus concentration methods to detect human norovirus (HuNoV) in water. One conventional virus concentration method using an electropositive filter (1MDS-method) and two methods developed by our research group using an electronegative filter (Mg-method and Al-...

journal_title：Journal of virological methods

authors： Haramoto E,Katayama H,Utagawa E,Ohgaki S

更新日期：2009-09-01 00:00:00

abstract：:Rapid and reliable detection of varicella-zoster virus (VZV) and herpes simplex virus type 1 (HSV-1) and -2 (HSV-2) is of clinical significance in immunocompromised patients and patients with infections of the central nervous system. This paper describes the detection of VZV and HSV using the commercially available Af...

journal_title：Journal of virological methods

authors： Finnström N,Bergsten K,Ström H,Sundell T,Martin S,Wutzler P,Sauerbrei A

更新日期：2009-09-01 00:00:00

abstract：:The diversity and extent of sequence variations between hepatitis C virus (HCV) isolates from Pakistan were studied and the probable effects of these variations were assessed on secondary viral structures. Sequencing and phylogenetic analysis was performed on 33 samples, of which 25 were typed as genotype 3 by RFLP (r...

journal_title：Journal of virological methods

authors： Yasmeen A,Siddiqui AA,Hamid S,Sultana T,Jafri W,Persson MA

更新日期：2009-09-01 00:00:00

abstract：:Manipulation of the coronavirus genome to accommodate and express foreign genes is an attractive approach for gene delivery and vaccine development. By using an infectious cloning system developed recently for the avian coronavirus infectious bronchitis virus (IBV), the enhanced green fluorescent protein (EGFP) gene, ...

journal_title：Journal of virological methods

authors： Shen H,Fang SG,Chen B,Chen G,Tay FP,Liu DX

更新日期：2009-09-01 00:00:00

abstract：:Adenovirus (Ad) vectors have been developed as human immunodeficiency-1 (HIV-1) vaccine vectors because they consistently induce immune responses in preclinical animal models and human trials. Strong promoters and codon-optimization are often used to enhance vaccine-induced HIV-1 gene expression and immunogenicity. Ho...

journal_title：Journal of virological methods

authors： Zhao C,Crews CJ,Derdeyn CA,Blackwell JL

更新日期：2009-09-01 00:00:00

abstract：:Herpes simplex virus type 1 (HSV-1) amplicon preparations are usually quantified as transducing units/ml (TU/ml), with little information on genomic copy/TU ratios. In the present study, two HSV-1 amplicons expressing enhanced green fluorescent protein (EGFP) were analysed by quantitative PCR (qPCR) and transducing ac...

journal_title：Journal of virological methods

authors： El-Sherbini YM,Stevenson MM,Seymour LW,Wade-Martins R

更新日期：2009-08-01 00:00:00